The research aimed to evaluate the influence exerted by the removal of the testicles (orhidectomy) in roosters, on the fatty acids profile. In this regard, were formed two batches of roosters belonging to the Transylvanian Naked Neck breed, one batch was experimental (Lexp), composed of 20 castrated birds at the age of 7 weeks, and one control batch (Lm) composed of 10 uncastrated roosters. The birds of the two groups were raised under identical conditions and received the same type of compound feed; their slaughter was performed at the age of 20 weeks. The results obtained after reporting the values ​​of saturated fatty acids to unsaturated fatty acids recorded the highest value, of 0.47, in case of the muscles from the upper thighs of roosters (Lm). Regarding the ratio between polyunsaturated fatty acids and monounsaturated fatty acids, the highest value, of 1.12, was calculated for the muscles of the upper thighs from capons (Lexp). Regarding the Ω3 / Ω6 ratio, the highest value, of 17.81, was calculated for the muscles of the upper thighs from the capons, while, at the opposite pole, was the result for the pectoral muscles of roosters from Lm, in value of 12.48. We recommend continuing research in this direction.