Andean berry (Vaccinium meridionale Swartz) or “Mortiño” is a tropical-origin commonly cultivated fruit from the Andean region of South America. The fruit is usually consumed in several preparations such as juice, jams, or fresh fruit, and contains a wide variety of phytochemicals, of which polyphenols account for the most abundant group. Previously, it has been demonstrated the chemopreventive and chemoprotective effects of the juice (ABJ) on colorectal cancer in vitroand in vivo, and its anti-inflammatory properties in vitro as well. However, still more research is needed elucidating molecular mechanisms linked to ABJ. This research aimed to assess the antiproliferative effects of ABJ on two representative human colon adenocarcinoma cells from primary (SW480) and secondary (SW620) tumors. Cells were exposed to several ABJ concentrations (0, 10, 20, and 30 % v/v), and their 24 h-viability was tested (MTT), while cell proliferation was assessed at 24, 48, and 72 h (Sulphorhodamine B). Cloning efficiency was tested using Carnoy’s solution staining. In silico molecular docking was tested using selected ABJ polyphenols (cyanidin-3-glucoside and chlorogenic acid), previously against molecular cancer targets. All treatments reduced cell viability (<80 %, p<0.05), being 30 % v/v were the most effective on SW620 cells (IC_50-SW480: 28.19 % v/v ABJ; IC_{50 SW620}: 19.43 % v/v ABJ). A similar trend was shown for the antiproliferative assay, but 20 % v/v ABJ at 24 h was the most antiproliferative treatment for both cell lines. After ABJ treatments, none of SW480 populations were positive for Carnoy’s stain. In silico analysis indicated the highest binding between PON2, Catalase, and Claspin (-9.4 kcal/mol) and cyanidin-3-glucoside, whereas HSP27 and PON2 (-8.7 and -8.3 kcal/mol) were the highest binding affinities for chlorogenic acid. Results indicated that ABJ exerts antiproliferative and inhibits cell viability on SW480 and SW620 cells, which could be attributable to interactions between its main polyphenols and apoptosis-related cancer targets.