The study examined how well the alkaloid content of Vitex doniana affected the liver function in albino rats. The liver damage in albino rats was induced using a CCl₄ solution in paraffin (1:1). The liver function tests were performed by administering several groups of albino rats oral dosages (200, 400, and 600 mg/kg) of the bulk alkaloid over the course of 14 days, After the rats were sacrificed, the liver was examined hepatopathologically to determine the serum levels of albumin, total protein, cholesterol, triglycerides, low-density lipoproteins (LDLs), and high-density lipoproteins (HDLs). When compared to the control, the alkaloid fraction of V. doniana significantly (p ˂ 0.05) enhanced the levels of total protein, serum albumin, and HDLs. The findings also demonstrated that rats administered with alkaloid fraction had significantly (p ˂ 0.05) lower serum levels of CHOL, TAG, LDLs, and BUN as compared to untreated rats. The alkaloids repaired the normal cellular architecture of the liver that had been damaged by the standard hepatotoxin. The use of V. doniana in treating disorders linked to the liver is supported by the fact that its alkaloids repair CCl₄-induced hepatocellular damage.