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Essential oils of Pulicaria odora L: chemical composition and effect on anti-aging gene expression in human keratinocyte cells
* 1 , 2 , 1 , 3 , 4 , 1 , 1
1  Laboratory of Bioorganic Chemistry, Department of Chemistry, University Chouaïb Doukkali, El Jadida, Morocco
2  Laboratory of Sustainable Development and Health Research, Faculty of Sciences and Techniques, Cadi Ayyad University, Marrakech, Morocco
3  Laboratory of Agri-Food, Biotechnology; and Valorisation of Plant Resources, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech
4  Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395. Japan
Academic Editor: Alfredo Berzal-Herranz (registering DOI)

In traditional Moroccan medicine, the roots of Pulicaria odora L. (PO) are used against menstrual cramps and intestinal disorders and are highly valued as a spice for their flavor. Several natural compounds are characterized by various biological properties such as antimicrobial, antiviral, antioxidant, anticancer, and anti-aging.

In this study, PO essential oil (EO) is evaluated for the first time for its effect i) on the HTERT gene, a catalytic enzyme that is required for telomerase activity, ii) on the human keratinocyte growth factor (KGF), a secreted protein that could play an important role in the repair of skin injury and that has also been implicated to play a role in other diseases, iii) and on the Sirtuin 1 (SIRT1) which plays an essential role in regulating the cell cycle and energy homeostasis. The EO of PO roots was obtained by hydrodistillation and analyzed by GC/MS. We used Quantitative reverse transcription-polymerase chain reactions (qRT-PCR) to determine the effect of the EO on expression levels of KGF, SIRT1, and HTERT genes in HaCaT cells.

We have identified 22 volatile compounds representing 93.76% of the oil by GC/MS. The oil was dominated by oxygenated compounds with about 93.32% against only 0.44% of terpene hydrocarbons. The KGF expression level in HaCaT cells exposed to EO is found to be significantly higher than resveratrol (RSV) (p<0.05). Also, the EO and resveratrol have induced a similar activity on HTERT and SIRT1 expression (p<0.05).

Keywords: Pulicaria odora, essential oil, antiaging, GC/MS, gene expression, HaCat cell