Enteroaggregative Escherichia coli (EAEC) has widely been recognized as one of the leading causes of infantile diarrhoea and nutrient malabsorption both in developing and developed countries. In recent times, drug resistance, particularly multi-drug resistance (MDR) among the EAEC strains has been documented and would result in a therapeutic stumble; antimicrobial alternatives are employed widely to curb this emerging public health menace. In the present study, a facile one-pot synthesis of silver/zinc oxide nanocomposites (Ag/ZnO NCs) using methanolic extract of stem and leaves of Curcuma longa was performed. The synthesis of Ag/ZnO NCs was confirmed using UV-vis spectroscopy (UV-vis) and Fourier transform infrared spectroscopy (FTIR), while the thermal stability was ascertained by thermogravimetric analysis with differential thermogravimetric analysis (TGA/DTA), whereas crystallinity was determined using Powder X-ray diffraction (PXRD). The shape and size of the green synthesized Ag/ZnO NCs determined using field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) revealed an irregular polycrystalline morphology with a size of 31.34 ± 1.27 nm. Later, the antibacterial potential of the green synthesized Ag/ZnO NCs evaluated against MDR- EAEC strains revealed a minimum inhibitory concentration (MIC) of 31.25 μg/mL and a minimum bactericidal concentration (MBC) ranging from 62.50 to 125 μg/mL. Moreover, the green synthesized Ag/ZnO NCs inhibited the biofilm-forming ability of the tested strains of MDR-EAEC. Besides, a concentration-dependent antioxidant activity was exhibited by the green synthesized Ag/ZnO NCs as evidenced by ABTS assay and reducing power assay. Overall, this study demonstrated the antibacterial, antifouling as well as antioxidant potential of green synthesized Ag/ZnO NCs using C. longa extract, which might be used as an alternative therapeutic candidate.