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Differences in the Expression Pattern of mRNA Protein SEMA3F in Endometrial Cancer in vitro under Cisplatin Treatment
* 1 , 1, 2 , 1, 3 , 4 , * 5, 6
1  Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academy of Silesia, 41-800 Zabrze, Poland
2  Hospital of Ministry of Interior and Administration, 40-052 Katowice, Poland
3  ICZ Healthcare Hospital in Zywiec, 34-300 Zywiec, Poland
4  Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, 41-800 Zabrze, Poland
5  Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia, 40-555 Katowice, Poland
6  Department of Neurosurgery, 5th Military Clinical Hospital with the SP ZOZ Polyclinic in Krakow, 30-901 Krakow, Poland
Academic Editor: Humbert G. Díaz

https://doi.org/10.3390/mol2net-09-14276 (registering DOI)
Abstract:

Semaphorin 3F (SEMA3F) plays a substantial role in carcinogenesis, because of its role in inducing angiogenesis, and creating a microenvironment for the developing tumor. The purpose of this work was to assess the impact of cisplatin, depending on the concentra- tion and exposure time on the expression pattern of SEMA3F in an endometrial cancer cell line. Cultures of the Ishikawa endometrial cancer cells were incubated with cispla- tin with the following concentrations: 2.5μM; 5μM; and 10μM and for the following periods of time: 12; 24; and 48 hours. Cells not incubated with the drug constituted the control in the experiment. To determine the effect of cisplatin on the expression of SEMA3F, the real-time quantitative reverse tran- scription reaction (RtqPCR; mRNA) was used, as well as the ELISA assay (protein). The statistical analysis was done with the admission of p<0.05. The silencing of SEMA3F expression on the transcriptome and proteome levels in a culture unexposed to the effects of cisplatin in comparison to endometrial cancer cells under the influence of cisplatin (p<0.05) were noted. Along with an increase in the concentration of the drug used, the num- ber of copies of the gene transcript, during the shortest incubation period had a gradual increase. Only for the highest concentration of the drug, substantial statistical differences in the expression of the SEMA3F protein between 24 and 48 hour incubation periods (p<0.05) were determined. Using cisplatin in an endometrial cancer cell culture results in an increased expression of SEMA3F, which advantageously affects the normalization of the neoplastic angiogenic process and lowers the proliferation of the cells making up the mass of the tumor.

Keywords: SEMA3F, endometrial cancer cell line, cisplatin, expression, supplementary molecular marker
Comments on this paper
Maider Baltasar Marchueta
Dear authors thank you for your support to the conference.

Now we closed the publication phase and launched the post-publication phase of the conference. REVIEWWWERS Brainstorming Workshop is now open until January 25th. MOL2NET committee, authors, and social media followers worldwide are invited to post questions/answers and comments about papers. Please kindly post your public Answers (A) to the following questions in order to promote interchange of scientific ideas.

My Questions (Q) to you:
Q1. Could you elaborate on the significance of the noted statistical differences in SEMA3F protein expression between 24 and 48-hour incubation periods
Q2. and how this information may guide future investigations or potential therapeutic strategies for endometrial cancer?

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