Background and Objectives: The international protocol used to diagnosis Non-Small Cell Lung Cancer (NSCLC) usually faced an inappropriate result due to the poor diagnostic ability in early stage. Carcinoembryonic Antigen (CEA), an established serum tumor markers that is using for NSCLC diagnosis, has a limited sensitivity and specificity, but still the predominant complementary tool detection in where its results is confirmed the diagnostic radiology finding (PET-CT). Unfortunately, the limited range of its sensitivity is unable to classify approximately one third of patients suffering from NSCLC. Due to a huge number of patients is lately classified as NSCLC; the efficacy of the offered treatment is limited. Hence, the importance of discover, improve, and establish a new technique that participates in the NSCLC diagnosis indeed urgent.

Methods: The low angle x-ray scattering (LAXS) technique was applied on the lyophilized serum of NSCLC patients to create patient profile that able to distinguish the molecular difference between NSCLC patients avoiding the undesirable radiation exposure to the patients.

Results: The created LAXS profile is characterized by two peaks. The first scattering peak at 4.8° is sensitive to molecular alteration in protein structure that is the main characteristic difference from normal serum. Comparing the measurements of LAXS profiles of NSCLC with normal sera; the unique first scattering peak at 4.8° is elucidated a characterization shape and profile for NSCLC and normal individuals.

Conclusion: Using LAXS technique gives us full details at molecular level that is introducing as a promising tool which could be a supporter in NSCLC early detection.