Development of bacterial resistance towards existing antibiotics is a universal problem for human and animal health as well as for food security. In an attempt to overcome this problem, nanotechnology has contributed with nano formulations. However, these are associated with risks and drawbacks including environmental toxicity, cell injury, issues of high production cost and scarcity of active ingredients. On the other hand, green synthesis of nano formulations by biological methods is a simple, innovative, ecofriendly, cost effective and advanced approach for the treatment of lethal infections caused by multidrug resistant organisms like staphylococcus aureus . About 30% of humans are asymptomatic carriers of S. aureus in their upper respiratory tract.Clinical disease caused by S. aureus infections range from mild to severe and may be manifested in the form of pneumonia, osteomyelitis, skin and deep tissue infections Here, we prepared plant-mediated gold nanoparticles from Camellia sinensis and Cocos nucifera. The green biocompatible nanoparticles were characterized by using UV-Visible spectroscopy (UV-Vis spectroscopy), X-ray diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscopy (TEM), Dynamic.light.scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). Moreover, these green gold nanoparticles were investigated for their antimicrobial activity by checking minimum inhibitory concentration (MIC). We found that the newly developed bionanoparticles showed strong activity against the multidrug resistant Staphylococcus aureus.