In this study, acetone extract from Trachyspermum ammi was prepared and used as the reducing agent for the eco-friendly synthesis of silver nanoparticles (AgNPs) by using silver nitrate solution. UV-VIS, FTIR, XRD and STEM were used to confirm the formation characterization of AgNPs. The measured average size of AgNPs was 10-25nm. The larvicidal activity of acetone extract and synthesized AgNPs were evaluated against the 2^nd and 3^rd instar larvae of Aedes aegypti and Ae. albopictus responsible for the diseases of public health importance. Larvae of Ae.aegypti and Ae. albopictus were kept in the different concentrations (50-250 ppm) of plant extract and green AgNPs to calculate the percentage mortality at time intervals of 6,12, 18, 24, 30, 36, 42 and 48h. The nanoparticles proved excellent larvicidal agents for the both Ae. aegypti and Ae. albopictus (dengue vectors) with LC₅₀ values of 76.28 ppm; 80.28 ppm for 2^nd and 87.02 ppm; 89.02 ppm for 3^rd instar larvae compared to the plant extract 121.44 ppm; 127.89 ppm for 2^nd and 127.89 ppm;129.35 ppm for 3^rd instar larvae respectively after 48 hours exposure time. Highest percentage mortality of T. ammi AgNPs against the 2^nd instar larvae of both Ae. aegypti and Ae.albopictus was recorded as 100% ± 0.1342 at the concentration of 250 ppm after 36h exposure time, while for 3^rd instar larvae, the highest mortality(100% ± 0.954) was achieved after the 48h. In case of T. ammi seed extract highest mortality was recorded as 100% ± 0.919; 90% ± 0.234 against 2^nd and 3^rd instar larvae of Ae. aegypti respectively after 48h exposure time and at 250ppm concentration, while for Ae.albopictus these values were 93% ± 0.887; 92% ± 1.034 after the same time and concentration. Our results suggest the extract of T.ammi and synthesized T.ammi AgNPs as excellent controlling agents for vector mosquitoes instead of pollution causing existing chemical pesticides