This overview concerns recent patents and patented technologies in relation to the development of hydrogel-based biosensors, published until 2022. As a result, 257 patent documents and 145 simple patent families have been searched through different specialized patent databases. Furthermore, the patent classification confirmed that the most claimed inventions concern chemical analysis of biological material and biospecific binding assay materials with an insoluble carrier for immobilizing immunochemicals. Overall, research, development, and innovation concerning hydrogel-based biosensors are based on improvements in the synthesis of hydrogels, biomolecule immobilization and detection, as well as microelectronic device integration and microfabrication techniques. A collection of recent patented technologies is proposed at the end. In this respect, it aimed to demonstrate potential trends and challenges in relation to the development of hydrogel-based biosensors.