In this work, we report the development of a technique to fabricate a screen-printed electrode (SPE) and its applications for uric acid sensing. The SPE was fabricated using a printing technique using an office printer printed on a photo paper substrate. Particularly, the conductive ink used for printing the working electrode (WE) and counter electrode (CE) consisted of graphene oxide (GO) and gold nanorod (AuNR) material. While the reference electrode (RE) was made by applying the conductive silver paste to the fabricated SPE. The electrochemical measurement of uric acid solution using fabricated SPE GO/AuNR provides a higher signal than the commercially available SPE. The electroanalytical performance of the fabricated SPE based on GO/AuNR toward the measurement of uric acid solution exhibited a linear range of 0.8−200 μM, a detection limit of 0.5 μM, a quantitation limit of 1.0 μM, outstanding repeatability (% relative standard deviation) of 4.885 % as well as good selectivity with ascorbic acid, dopamine, glucose, urea, and sodium as interference. Furthermore, the fabricated SPE based on GO/AuNR was successfully employed for the determination of uric acid concentration in human urine samples using the standard addition approach.