Aminonitriles are substances frequently used to obtain other compounds. This circumstance raised the hypothesis that there was intrinsic to such structures relevant therapeutic potential to be scientifically explored. From this perspective, certain studies have identified that the performance of this group of molecules go beyond their participation only as an intermediary of new pharmacological formulations and demonstrate their own antibiotic evidence. In the face of studies carried out to support this research, it was observed that the potential of aminonitrils covers the fight against fungi, parasites, bacteria and even tumors. In this context, the present work aimed to carry out a screening of the antibacterial activity of substituted aminonitriles. Therefore, the disc-diffusion method was used to assess the antibacterial activity of seven new molecules of substituted aminonitrils (HAN-1 to HAN-7), at concentrations of 16, 32, 64, 128, 256, 512 and 1,024 µg /mL, against gram-positive bacteria Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212, and gram-negative bacteria Pseudomonas aeruginosa ATCC 27853; Proteus mirabilis ATCC 25933 and Escherichia coli ATCC 25922. After incubation, it was observed that none of the tested molecules were able to form bacterial growth inhibition halo. Thus, it is necessary to continue investigating the antimicrobial potential of this group of compounds using more sensitive methodologies to confirm or rule out their bioactivity in these microorganisms.