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Ultrasensitive Raman spectroscopy-based virus detection usingglycan-coated plasmonic substrates
* 1 , 2
1  Teesside University, United Kingdom
2  Shizuoka University, Japan
Academic Editor: Nicole Jaffrezic-Renault

Abstract:

Hepatitis viral infections are the most common cause of hepatitis liver disease, which eventually leads to cancer and fibrosis if undetected early. Therefore, early detection would allow for pre-ventive and therapeutic actions. Here, a Raman spectroscopy-based biosensor was developed using plasmonic molybdenum trioxide quantum dots-based substrates functionalized with a proteoglycan (Syndecan-1) as a novel bioreceptor for target hepatitis E virus (HEV). The innova-tive bio-detection system achieved a detection limit of 1.05 fg/mL for tested HEV antigen (ORF2), indicating superb clinically relevant sensitivity and performance. The designed biosensing system incorporating a glycan motif as a bioreceptor instead of the conventional antibodies or aptamers, presents new insights for the ultrasensitive detection of HEV and other infectious viruses.

Keywords: Raman; biosensors; virus detection; glycans; nanogels

 
 
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