Biodiesel is defined as a mixture of fatty acids and methyl esters. Using waste cooking oils as raw material can prefer an acidic catalyst to carry out an esterification reaction in biodiesel production. Researchers have generally used heterogeneous sulfonated solid catalysts for this reaction. However, the by-product water, the result of the biodiesel production process, absorbs on the catalyst surface. So, it is crucial to select hydrophobic support. To use sulfonated UiO-66 for biodiesel production via the esterification of waste cooking oil is logical. Besides its hydrophobicity, it knows that UiO-66 has high chemical and thermal stability, high surface area, and uniform pore structure. The study aims to determine the effective parameter in biodiesel synthesis from oleic acid esterification on sulfonated UiO-66 with ANCOVA analysis. For optimization analysis, Sigma Plot 14.0 software was utilized as software. Holm-Sidak test was applied for one-way ANCOVA. Parameters were effective on the oleic acid conversion rate selected as esterification temperature, time, catalyst amount, and methanol/oleic acid rate.