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Development of an LC-MS/MS-based method for quantification Tdp1 inhibitor based on usnic acid and distribution study in tissue of healthy and tumor mice
* 1, 2 , 3 , 1, 2 , 1 , 2, 4 , 4 , 3 , 3 , 5 , 5, 6 , 2, 3
1  N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, 630090, Lavrent’ev ave., 9, Novosibirsk, Russia
2  Novosibirsk State University, Pirogov St. 2, 630090, Novosibirsk, Russia
3  Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of Russian Academy of Sciences, 630090, Lavrent’ev ave., 8, Novosibirsk, Russia
4  Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090, Lavrent’ev ave., 10, Novosibirsk, Russia
5  Novosibirsk State University, 630090, Russia, Novosibirsk, Pirogova, 1
6  Vorozhtsov Novosibirsk Institute of Organic Chemistry, 630090, Russia, Novosibirsk, Lavrentjev prosp., 9
Academic Editor: Alfredo Berzal-Herranz

Published: 01 November 2023 by MDPI in 9th International Electronic Conference on Medicinal Chemistry session General (registering DOI)

Tyrosyl DNA phosphodiesterase 1 (Tdp1) is a promising target for the treatment of cancer, since it plays a key role in the removal of DNA damage generated by the action of topoisomerase 1 (Top1) poisons (irinotecan and topotecan) widely used in anticancer therapy. We have previously shown that the Tdp1 inhibitor, enamine derivative of usnic acid, the agent OL9-116, enhances the antitumor activity of topotecan and studied the pharmacokinetics of the agent. In the present study, we developed and validated an LC-MS/MS methods for the quantification of OL9-116 in mouse lungs, liver, kidneys, Lewis lung carcinoma tumor nodes and studied its distribution in organs of healthy and tumor mice. QuEChERS methodology was selected for sample preparation. Quantification of the compound was performed using SCIEX 6500 QTRAP mass spectrometer in MRM mode following chromatographic separation on a C8 reversed-phase column. Pharmacokinetics of OL9-116 was studied after intragastric administration of the compound to healthy and tumor mice at a dose of 150 mg/kg. It was found that maximal concentration of the agent in organs of healthy mice can reach 100 µg/g followed by its distribution and excretion. In tumor mice we observed increasing concentration of OL9-116 during first 1 h after administration to a level of 5-30 µg/g depending on the tissue (lungs, liver, kidneys, tumor node) and remaining at this level for at least next 12 hours, thus showing a pharmacokinetics profile clearly differing from that of healthy animals.

Keywords: antitumor activity; LC-MS/MS; QuEChERS methodology; pharmacokinetics; tissue distibution; tyrosyl DNA phosphodiesterase 1; usnic acid derivative