The goal is to develop a throat spray containing Bacillus and ivy leaves extract that prevents and aids in the treatment of pharyngitis and upper respiratory tract infections. JMP 14 Pro software was used to create an experimental model using 16 formulas. The percentages of ivy leaves extract, honey, and Bacillus spores are independent variables, while Bacillus survival and mean beam width spray are dependent variables. Based on the variance analysis of the dependent variables and the effect of the independent factors on the dependent variable, the computer creates optimum equations with various desired values. The assessment of appropriate formulae by examining anti-inflammatory efficacy as well as quality standards such as organoleptic, pH, qualitative ivy leaves extract, and Bacillus survival quantification. The findings demonstrated that JMP 14 Pro suggested an ideal formula with an index that was the most preferred and contained 1,65% ivy leaves extract. The survey approach showed that the formula had good anti-inflammatory power when compared to the reference drug. The anti-inflammatory potential was evaluated using the mouse paw edema test on white mice. The end product is a suspension with a mild and sweet flavor. It achieves a constant spray volume, with a pH range of 5,5 to 6,5, and a round or almost circular spray shape that measures 7,2 to 9,3 cm in diameter. It also matches the standard's Rf on thin layer chromatography (HPTLC). Strong anti-inflammatory properties are present in the optimal formulation of the Bacillus-containing throat spray, and the quality criteria are studied as a basis for the eventual establishing of the product's quality standards.