Bacteroides xylanysolvens is a gram-negative, anaerobic rod alongside xylan-degrading bacterium identified from human feces samples. An In silico technique can help us better comprehend the uncharacterized protein of Bacteroides xylanysolvens (accession ID: CUN90054) by examining its functional annotations, identification, and structural characterization. In silico pathways are used to investigate the protein's physiochemical properties, functional annotation, structure prediction, active site analysis, and sub-cellular localization. The annotated hypothetical protein is connected to Metal-independent alpha-mannosidase (MIAM) including both bacterial and fungal glycoside hydrolases (GH125), according to the current study. This finding may be of considerable relevance to future bacterial genetics research.