Lemon balm (Melissa officinalis L.) is a medicinal and aromatic plant (MAP) belonging to the Lamiaceae family. Thanks to the high amount of secondary metabolites, such as phenolic compounds, flavonoids, and essential oils in the aerial parts, this species is a well-known herb used for different pharmaceutical, food/beverage, and cosmetic purposes. Like other MAPs, lemon balm shows significant variations in productive and qualitative parameters due to the effect of biotic and abiotic factors. The quantity of secondary metabolites represents a marker for MAP quality evaluation. The aim of this study was to assess the effects of foliar treatments with four different commercial biostimulants (B), namely Eklonia maxima, Ascophillum nodosum, fulvic acid, and protein hydrolysates, as well as two application frequencies (F) based on productive and qualitative parameters of lemon balm under organic agriculture conditions. The control treatment was provided by water only. After harvest, a number of parameters, such as plant height, total fresh yield, total dry yield, total phenolic, antioxidant activity, and rosmarinic acid levels, were measured. Morphological, productive, and qualitative traits were affected by both experimental factors and their interaction. The highest plant height was observed in plants exposed to protein hydrolysates. The highest fresh and dry yields were obtained in plants treated with fulvic acids applied every two weeks. The highest phenolic content was found in plants treated, weekly, with fulvic acids and protein hydrolysates. The highest antioxidant activity was recorded in plants treated every two weeks with E. maxima-based seaweed extract. Overall, this study represents an important step towards the organic cultivation of MAPs.