Regenerative medicine is a promising field that aims to reconstruct diseased or defective tissues. The primary goal of tissue engineering is to develop systems that can mimic the natural ECM using both natural and synthetic materials, and nanofibers are an excellent tool for this purpose. Electrospinning is a method for fabricating fibrous structures that offer great potential for the creation of biomimetic systems. Nanofibers mimic key aspects of the native ECM, making them a promising approach to improving tissue efficiency. Collagen and other extracellular matrix fibrous proteins with diameters in the nanometer or sub-micrometer range are abundant in the body, and porous nanofibers with high surface area and porosity can be used to mimic the ECM and promote tissue regeneration. Advanced bioactive dressings can be made using electrospinning. These dressings are popular due to their flexibility, ability to mimic the structure of the extracellular matrix (ECM), and ability to support the wound healing process. This emerging technology has the potential to create effective wound dressings and care products. There is a great focus on the development of suitable fibrous bioactive dressing materials for the treatment of chronic and acute wounds. Various composite fibers made from natural and synthetic polymers are used to create these dressings. Additionally, medicinal and biological agents are incorporated into the structure of fibrous dressings to control pain, prevent infection, and promote tissue repair and regeneration. This ensures that the wound-healing process progresses smoothly without any possible complications. As innovation progresses, more complex systems are developed in a controlled manner.