Introduction. The development of new strategies to repair large segmental bone defects is currently an ongoing challenge all around the world, and biomaterials suitable for dealing with this are in high demand. An important aim in this field is to achieve simultaneously both the mechanical and biological requirements of the implant site.

Methodology. In this study, we propose obtaining a bioinspired bone tissue substitute using the stiff and modulable mycelium of Ganoderma sessile. The mycelium was cultured on a substrate composed of alginate crosslinked by hydroxyapatite nanoparticles (ALG-HAn), with in vitro osteogenic properties previously verified by the authors. Then, the mycelium was inactivated and sterilized by autoclaving to obtain the final biomaterial.

Results. Using scanning electron microscopy (SEM), it was possible to confirm that the mycelium acts as a directing agent of the biomaterial microstructure. The mycelium colonized the ALG-HAn substrate, leading to the formation of a trabecular bone-like network with a hierarchical structure. Moreover, static water contact angle assays demonstrated that the presence of ALG in the membranes significantly reduced the hydrophobicity of the biomaterials. Finally, to test the interaction between blood cells and biomaterial, we verify the lack of hemolysis in human plasma samples.

Conclusion. The promising results of this work will provide a new perspective for the future development of mycelium-based biomaterials applied for bone tissue regeneration.