Recently, studies have been conducted utilizing the pellicle of Nata de Coco (NC) as a sustainable source of bacterial cellulose (BC) and Squid Gladius (SG) for chitosan. In this work, the synthesis of a biocompatible bacterial cellulose–chitosan composite (BCC) from NC and SG was conducted. In addition, the effects of varying chitosan concentrations (2%, 5%, 8% w/v) on the physicochemical properties (morphology, water holding capacity, and tensile strength) and biocompatibility were investigated using Simulated Body Fluid (SBF). The BCC composite showed a denser and more homogenized structure compared to the BC pellicle, as was seen from the scanning electron microscopy (SEM). The Fourier Transform–infrared spectroscopy (FTIR) results demonstrated that the spectra of the pellicles were in the 2800 to 1200 cm-1 wave range, while the bands for the amide groups were observed at peaks at 1613, 1550, and 1337 cm-1, which were associated with the presence of chitosan. The highest water holding capacity of 581% was achieved by 8% w/v chitosan containing BCC, while the 5% w/v chitosan containing BCC tended to endure more tensile force. The SEM images of the BCC pellicles that had undergone the SBF process showed the gradual progress of the formation of the hydroxyapatite (HaP) crystals with a Ca-P ratio of 1.67 on the third day, as revealed by the X-ray Fluorescence (XRF) indicating a biocompatible property. The FTIR analysis showed all the typical absorption characteristics of HaP, ranging from bands observed at 3555 and 622 cm-1 due to the stretching mode of hydrogen-bonded ions and liberational mode of hydrogen-bonded ions, respectively. The results of this study showed the potential of producing biopolymers from biomass for possible wound dressing products for biomedical applications.