Resveratrol is a natural polyphenol found in many vegetables and fruits. Scientific reports have demonstrated its broad pharmacological activity, including cardioprotective, anti-cancer, anti-microbial, anti-ageing, antioxidant, immunomodulatory and neuroprotective effects. However, due to its low solubility, as well as its sensitivity to light and elevated temperature, it is susceptible to configuration transformation and rapid degradation. Various analytical techniques are used to assess the stability of solid drug forms. The aim of this work was to assess the stability of tablets containing this natural polyphenol, after storage under stress conditions, using hyperspectral analysis (THR). To test the suitability of THR to monitor the stability of the tablets under study, the formulations were stored in a Solarbox 1500 chamber (COFOMEGRA Srl, Milan, Italy) for different periods of time (1 day, 5 days) at two different temperatures (20⁰C, 45⁰C), yielding four storage conditions. The photostability test was performed in accordance with ICHQ1B guidelines. SOC-410 Directional Hemispherical Reflectometer (Surface Optics Corporation, USA) was used to determine the total reflectance of tested tablets bands in the spectral range of 335–2500 nm. Twenty tablets were tested at each time point, and the results obtained were subjected to statistical analysis. Irrespective of storage conditions, significantly lower mean directional reflectance was observed for tablets stored under stress conditions compared with the values determined at the beginning of the experiment (0 day). Furthermore, the highest total directional reflectance was observed for the two near-infrared spectral ranges (700-1100 nm and 1000-1700 nm), while the lowest was observed for the near-ultraviolet range (335-380 nm). The present study showed that during storage under stressful conditions (heat and UV radiation), more light was transferred to the interior of the tablet matrix, which may have been due to changes in its physico-chemical parameters.