Pseudomonas taetrolens is a Gram-negative bacterium renowned for its diverse metabolic capabilities, making it a significant subject of study in biotechnological and industrial processes. Research on Pseudomonas taetrolens often aims to optimize conditions for the production of valuable biochemicals, elucidate its metabolic pathways, and explore its potential in industrial applications. This bacterium is particularly noteworthy for its roles in biotransformation and bioremediation, which are crucial in both environmental microbiology and industrial biotechnology. This study investigated the ability of Pseudomonas taetrolens to convert lactose into lactobionic acid using sweet and acid whey as substrates. The experimental setup involved controlling parameters such as temperature, pH, agitation speed, oxygen supply, and the viability and quantity of added bacterial biomass. The process was conducted in a bioreactor, with data collection every three hours and efficiency tested using high-performance liquid chromatography (HPLC). The results indicated that, under identical fermentation parameters, lactose was completely converted into lactobionic acid in sweet whey. In contrast, acid whey required repeated additions of Pseudomonas taetrolens biomass to achieve complete lactose conversion. These findings suggest that sweet whey is more suitable for lactose oxidation. However, further tests are necessary to conduct an in-depth analysis of the compositions of sweet and acid whey and their effects on the reproduction of Pseudomonas taetrolens.