Due to the side effects and resistance of pathogenic microorganisms to antibiotics and most drugs on the market, a great deal of attention has been paid to extracts and biologically active compounds isolated from plant species used in herbal medicine. For this purpose, we investigated two Artemisia species, with different tissues and from different years of vegetation. The growing year of the plants positively influence the activity of S. aureus, in the sense that MIC and MBC values for extracts from Artemisia absinthium L. growing year I are higher than those from growing year II. As a physiological part of the plant, the leaf, which is richer in compounds, has higher antimicrobial activity compared to the stems, and the second year of vegetation also positively influences the bacterial inhibition activity. A. absinthium leaf extract from the second year of vegetation showed higher antioxidant capacity as a Trolox antioxidant capacity equivalent, and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, in correlation with flavonoid and phenolic acid contents. So, based on the most recent literature, we can hypothesize the possible application of new therapeutic protocols for resistant infectious diseases, based on the use of natural extracts of Artemisia. Artemisia species with high biological values are a potential source of health and well-being .