Crustaceans represent an important group of macrofauna benthic in the coastal marine ecosystems, constitute important linkages in the food web, and can respond nonlinearly to environmental changes. Crustaceans’ diversity of the Gulf of Gabès was studied in eight localities in southern Tunisian coasts during the last years (2019--2024), and Benthic crustaceans sampling in the southern Tunisian coasts was carried out using a Van Veen grab, covering an area of about 0.05 m². In total, 20,114 individuals belonging to 262 crustacean species were identified. Amphipoda (48% total species), Decapoda (23%), and Isopoda (16%) are the most dominant taxa. Seagrass meadows (Posidonia oceanica, Cymodocea nodosa, Zostera noltei) constitute a suitable habitat for crustacean species in the Gulf of Gabès and include high diversity. The multivariate analysis (MDS, ANOSIM,..) revealed significant differences in the composition and trophic diversity of crustaceans' assemblages among the eight studied sites, and showed that the crustaceans’ distribution in the southern Tunisian coasts was correlated by many natural and anthropogenic factors, such as depth, sediment type, organic matter, and pollution gradient. This study suggests initiating a long-term monitoring program to improve our understanding of the temporal changes of crustacean communities in the Gulf of Gabès to recommend the necessary conservation measures in this area of high-value natural heritage.