The regulation of the orientation of a flying aircraft under autopilot is a multifaceted and crucial task that requires accuracy and flexibility. To do this, it is essential to have a complex control system that is furnished with an advanced controller capable of actively monitoring and modifying the flying characteristics of the aircraft. This must possess the ability to react dynamically to a range of disturbances experienced throughout the flight, including turbulence, fluctuations in wind, and other pertinent environmental elements. Through real-time adjustment of the flying attitude, the control system guarantees that the aircraft maintains its planned trajectory, stability, and safety along the whole trajectory. Typically, PID controllers are used to regulate the longitudinal direction of flights. However, these offline tuned controllers lack automation and are unable to adjust parameters in response to inherent disturbances seen in practice. Thus, this paper proposes online tuning techniques that are created using artificial intelligence (AI) mechanisms such as fuzzy logic and neural networks. The philosophy involved in this work is the online tuning of PID gain parameters by applying both aforementioned intelligent methods. The study also implements many traditional PID tuning techniques and compares the most effective tuning method with online approaches. To evaluate the effectiveness of online controllers and the optimal traditional PID controller, these controllers are subjected to different disturbances, and their performance is evaluated based on time-domain transient characteristics. The analysis revealed that the intelligent fuzzy controller-based PID controller outperformed alternative tuning techniques in terms of time performance indices such as delay time, rise time, peak time, and settling time, which are improved by 5.88%, 3.26%, 8.05%, and 55.71% respectively when compared to traditional PID tuning methods. The overall comprehensive analysis is conducted using MATLAB/Simulink, and the most optimal online tuning approach is recommended for the controller design.