An investigation was conducted using protein hydrolysates (PHs), obtained from the enzymatic breakdown of animal and plant wastes, which contain active compounds showcasing biostimulant effects that can help in increasing growth, yield and quality. The study location of the field experiment was the Agricultural Research Farm, Banaras Hindu University, during the kharif season, n 2021—22. The treatments consisted of 0.5, 0.625, 0.75, 1 and 1.5 L/ha PH applied at 15 and 50 days after transplanting (DAT), along with an untreated control. Various treatments significantly influenced the growth parameters, including plant height, no. of tillers, and dry matter accumulation, and the yield parameters, including the number of panicles, the panicle length and weight, the number of filled grains per panicle, fertility percentage, and grain and straw yield. PH at 1.5 L/ha yielded the highest values, followed by PH at 0.875 L/ha, when compared to the untreated control, and the percentage increase in yield over untreated control increased linearly with a range of4.1% to 15%. Similarly, the protein hydrolysates at 1.5 L/ha showed higher nutrient content and a higher uptake of nitrogen, phosphorus, and potassium in the grains. The analysis of the provided results indicates that the most successful method for enhancing rice yield and quality in Varanasi's India agroclimatic conditions was to incorporate protein hydrolysates at a rate of 1.5 L/ha at 15 and 50 DAT.