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Assessment of gene signature deriving from prostate cancer-associated fibroblasts (CAFs)
* 1 , 1 , 2 , 1 , 1 , 1 , 1 , 1 , 3 , 4 , 1 , 1
1  Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy
2  Department of Cultures, Education and Society Department, University of Calabria, 87036 Rende, Italy
3  Division of Urology, Department of Surgery, Annunziata Hospital, Cosenza, 87100, Italy
4  Breast and General Surgery Unit, Annunziata Hospital Cosenza, Cosenza, 87100, Italy
Academic Editor: Alexander E. Kalyuzhny

Published: 21 March 2025 by MDPI in The 3rd International Online Conference on Cells session Cellular Signaling
Abstract:

Background: The tumor microenvironment plays a pivotal role in shaping tumor aggressiveness and driving disease progression. In this context, the identification of gene signatures characterizing cancer-associated fibroblasts (CAFs) obtained from the two most frequently diagnosed cancers worldwide, such as breast and prostate tumors, may improve outcome prediction and therapeutic strategies for patients.

Methods: The transcriptomes of CAFs isolated from breast and prostate cancer biopsies were analyzed using RNA sequencing. Data from The Cancer Genome Atlas (TCGA) were used to compare the gene expression profiles of CAFs with those of breast and prostate cancer patients. The clusterProfiler package was employed to perform pathway enrichment analysis, while the gene signature associated withprostate CAFs was identified by applying K-means clustering. Kaplan--Meier curves and log-rank tests were used to assess the prognostic significance of the signature in prostate cancer patients. A decision-tree classification approach validated the clustering results and the prognostic relevance of the gene signature.

Results: The comparisons of either breast and prostate CAFs transcriptomes or the gene expression landscapes of breast and prostate cancer patients allowed us to construct a gene signature counting 11 genes (IL13RA2, GDF7, IL33, CXCL1, TNFRSF19, CXCL6, LIFR, CXCL5, IL7, TSLP, and TNFSF15) with clinical implications in prostate cancer. Notably, the aforementioned genes are implicated in immune-related transduction pathways. Thereafter, clustering and classification analyses revealed that prostate cancer patients exhibiting low expression levels of these 11 genes are characterized by a worse prognosis with a high prediction accuracy.

Conclusions: The prostate CAFs-related gene signature identified here might serve as a prognostic indicator and might offer a valuable set of biomarkers for improving the management of prostate cancer patients.

Keywords: Prostate cancer; Cancer-associated fibroblasts (CAFs); Gene signature; K-means clustering;
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