The combination of low water requirements and exotic fruit production makes vine cacti a promising crop for developing agricultural economies in semi-arid regions. This research aimed to develop autopolyploid plants through the application of two antimitotic agents (colchicine and oryzalin) to both germinating seeds and axillary vegetative buds. The diploid Hylocereus Cultivar 04.25, S.91, 70.11.04, 98.338 and GA.127 were studied. Preliminary work using a water solution with dimethyl sulfoxide (DMSO) as concentrations as low or lower than 0.1%(w/v) with the triton and Colchicine were treated in auxillary vegetative buds. Survival rate were recorded and calculated after 30 days of the treatment and exposure periods. Flow Cytometery Analysis was performed in that analysis average value of S.91 is 6.30381, 04.25 - 5.24041, 04.25 - 5.67403 the chimera effect of 3n is produced yet still more samples to be analyzed. This study documents the successful creation of artificial autopolyploids in vine cactus species, generating important new resources for future advanced breeding efforts.