Portable real-time detection of Bisphenol A (BPA) in plastic-bottled water and large-scale milk samples presents significant challenges. Analytical techniques must prioritize specificity and sensitivity for accurate analysis. Affordability and rapid detection are also crucial to ensure practical application. Additionally, sustainability is essential when analyzing plastic-bottled water and large-scale water samples. The imaging sensor for smartphone-based portable CL imaging has significant potential for point-of-care applications. When combined with a smartphone readout device, it enables convenient and efficient environmental sensing, which makes it a promising tool for real-time detection in various environmental contexts. We designed and made the 96-well plate for CL image sensing of BPA by hydrothermally synthesizing a mixed-ligand MOF on the surface, characterized byspectrochemical characterization. With luminol, NaOH, H₂O₂, and BPA, the modified plate demonstrated high selectivity and tolerance for BPA. It exhibited distinct colour changes in the chemiluminescence (CL) image. Additionally, it was compatible with diluted plastic water and milk samples containing varying levels of BPA. Compared to conventional liquid chromatography–mass spectroscopy techniques, the modified plate yielded CL images at 1 pg/mL with recoveries exceeding 90% (n = 3), serving as a demand-driven alternative imaging device that eliminates the need for an additional light source and simplifies the basic operating system design essential for point-of-care biomedical diagnosis.