This study aimed to assess the occurrence of aflatoxin B₁ (AFB₁) and the total amount of aflatoxins (AFs) in commercial sweet potato and ready-to-eat sweet potato samples, collected from Punjab, Pakistan. A total of 480 commercial sweet potato and 365 ready-to-eat sweet potato samples were analyzed using HPLC. The results showed that 28.5% and 19.7% of commercial sweet potato and ready-to-eat sweet potato samples were found to be contaminated with AFs. The highest mean level of total AFs was 28.5 ± 4.2 µg/kg in commercial sweet potato samples from Daska (city), and the highest mean level in ready-to-eat samples of sweet potato was 14.50 ± 2.50 µg/kg, from the same city. The results showed that 40% of samples have total AF levels in the range of 1-10 µg/kg in commercial sweet potato samples (Jamki), and 13.3% of samples have total AF levels above 20 µg/kg from the city (Kila Soba Singh). The highest mean dietary intake levels of 3.16 µg/kg body weight/day were found in female volunteers in the age group 21-30 years. The results emphasized the need to implement effective storage practices that could be beneficial in minimizing the exposure of AFs to crops. Furthermore, the results would help implement strict laws for aflatoxins in food and food products.