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Synthesization, Characterization, and in vitro Evaluation of Cytotoxicity of Biomaterials based on Halloysite Nanotubes
* 1 , * 2 , * 1 , * 2 , * 2 , * 2
1  Tecnológico de Monterrey
2  Universidad de Monterrey

Abstract: Halloysite is aluminosilicate clay that has been used in controlled drug delivery, bone implants,nanocomposites and for the capture of flowing tumoral cells. In this study halloysite clay nanotubes (HNTs) were functionalized by two organosilanes, Trimethoxy(propyl)silane and Triethoxy(octyl)silane. Functionalization was carried out by heating HNTs and organosilanes at 55°C for 48 h. The suspension was vacuum filtered to obtain a solid phase. Untreated and modified samples were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR). Samples were added to different cell cultures for cytotoxicity evaluation. Biocompatibility and cytotoxicity of materials was determined using C6 rat glioblastoma and HEK (Human Embryonic Kidney) cells. These studies allow the identification ofpotential applications in biomedical areas.
Keywords: halloysite, cytotoxicity, functionalization
Comments on this paper
Marinella Sandros
surface functionalization
It seems the modification of HNTs is a long process, is there a possibility to do it in a shorter period?

How reproducible is your surface functionalization?
Antonio Sanchez
Sure it is, the process can be shortened by reducing the activation energy of the chemical modification on the effect of temperature; it can maintain the reaction under reflux. Moreover, much depends on the environment in which the chemical modification is performed. Kinetic studies could be performed by varying the temperature and solvent effects inclusive.

By using the same commercial clay, we guarantee the reproducibility of the chemical functionalization. It also depends on the reaction temperature, both as the solvent and the functionalizing reagent, in this case the reactive organosilane. However, comprehensive studies can be made to have a range that includes several nanoclays. Studies which are not the focus of our work.

Thank you very much for your questions, and we hope we have answered them in an accurate way.

Alexis Goytia
Why after you made that functionalization with the organosilanes the cytotoxicity becomes active?
Ana Karen Mendoza
According to the studies chitosan is the material that was cytotoxic either alone or with functionalized halloysite. Further studies will be able to determine the reasons of this.