Ibuprofen is a potent non-steroidal and anti-inflammatory drug derived from propionic acid, and its effects are brought about by the effective inhibition of the synthesis of both Cyclooxygenase isoforms COX-1 and COX-2. The main effects of ibuprofen are related to the effective control of moderate pain, acute inflammation and fever in many diseases such as Patent Ductus Arteriosus, dental pains, orthostatic hypotension, cystic fibrosis, rheumatoid arthritis and osteo-arthritis. Ibuprofen is widely used to reduce mild and moderate aches and pains and reduce fever and manage dysmenorrhea. It is very commonly used for the relief of acute symptoms such as fever and tension headaches. It is also used to manage mild to moderate pain and moderate to severe pain as an adjunct to opioid analgesics. The main aim of this research was to exactly quantify pure ibuprofen in a pharmaceutical, using a new developed spectrophotometric method in the ultraviolet (UV) range. Maximum absorption wavelength was found at λ = 227 nm, corresponding to the absorbance of A = 0.7469, relative to absolute methanol used as a control. The amount of pure ibuprofen calculated in the pharmaceutical was 395.398 mg, corresponding to 98.85% percentage content, and was very close to the official reference value (400 mg). The average percentage deviation of the content found from the stated official amount (400 mg) was 1.15%, below the maximum percentage limit (± 5%) allowed by the European and International Pharmacopoeias Rules.