This work presents a preliminary investigation into the development of electrospun nanofibers composed of polyacrylonitrile (PAN) blended with a bio-based polymer. The aim is to explore the feasibility of combining a synthetic, process-stable polymer with a renewable, potentially biocompatible component to create hybrid nanofibers suitable for various biomedical applications. Various blend ratios were prepared and processed using electrospinning to evaluate general spinnability, fiber formation, and morphological characteristics. Scanning Electron Microscopy (SEM) was employed for surface analysis, showing continuous, bead-free fibers with morphology influenced by the composition and nature of the polymer blend. The integration of a bio-based polymer is expected to improve the material’s biological performance while maintaining the structural benefits of PAN, offering a significant advancement in dual-functionality nanofibers. These nanofibrous materials could be relevant for future applications in fields such as wound care, filtration, or tissue-related systems, providing a promising avenue for further innovation. At this stage, the study focuses on the basic processing and structural characteristics, serving as a foundation for further investigation and development. Planned future work includes extended physicochemical characterization and comprehensive evaluation of application-specific properties, essential for practical deployment and usability. This approach supports the development of adaptable, functional nanomaterials based on sustainable components for future medical or bioengineering use, thus contributing to more sustainable and eco-friendly solutions in nanotechnology.