Cadmium (Cd) is a non-essential and toxic metal with high mobility in soils and strong bioaccumulative properties in fungi. This study presents novel data from an extensive biomonitoring campaign of wild mushrooms collected from 22 urban and rural locations across Leicestershire, UK. A total of 106 mushrooms, representing 14 species, were sampled and analysed by ICP-MS following acid digestion. Species identification was confirmed via DNA barcoding. Cd was detected in 78% of the samples, with concentrations ranging from below the detection limit (0.08 mg/kg) to 13.45 mg/kg dry weight. Contrary to expectations, Coprinus atramentarius (poisonous) showed the lowest Cd accumulation, while the edible species Agaricus bitorquis exhibited the highest levels (mean: 4.05 mg/kg). Geospatial analysis revealed that mushrooms from the North-West (2.75 mg/kg) and South-West (2.70 mg/kg) quadrants of Leicester contained significantly higher Cd levels compared to the South-East (0.64 mg/kg; p < 0.05). In A. bitorquis, caps accumulated significantly more Cd than stipes (4.05 vs. 2.20 mg/kg; p < 0.001), confirming species-specific tissue partitioning patterns. When benchmarked against the EU Maximum Allowable Concentration (MAC) for Cd in cultivated mushrooms (0.2 mg/kg), 47.2% of urban samples exceeded this regulatory threshold. Nevertheless, updated human health risk assessments (Hazard Quotient and Lifetime Cancer Risk) indicated no significant non-carcinogenic or carcinogenic risk from occasional consumption by either adults or children. These findings expand on previous moss-based biomonitoring studies in Leicester by directly demonstrating cadmium uptake in fungi growing in the same urban hotspots. The results reinforce the value of wild mushrooms as cost-effective sentinels for environmental metal(loid) pollution and underscore the need to integrate multiple bioindicator species in public health and soil management frameworks. Future research should explore seasonal variation, bioavailability, and co-exposure effects with other toxic elements.