Optimizing nitrogen (N) management in corn (Zea mays L.) production is critical for enhancing sustainability and profitability, especially given its non-nitrogen-fixing nature and the inefficiency of conventional N fertilization due to losses. This study, conducted in 2023 and 2024 at the Tidewater Agricultural Research and Extension Center (TAREC), evaluated the effectiveness of the microbial nitrogen product Pivot Bio® under irrigated and non-irrigated environments. A split-split plot design with three replications was used to assess four N rates (133, 200, 267, and 333 kg N ha^-1), four seeding rates (59,000, 74,000, 89,000, and 104,000 plants ha^-1), and the presence or absence of Pivot Bio®, which allowed a 45 kg ha^-1 N reduction in the biological treatments. Results revealed significant main effects of irrigation, nitrogen rate, and seeding rate on grain yield (p < 0.05), with the highest yield (12,668 kg ha^-1) observed under irrigated conditions with Pivot Bio® and 333 kg N ha^-1. Notably, the interaction between nitrogen and irrigation, seeding rate and irrigation, and nitrogen and seeding rate significantly influenced yield. Under non-irrigated conditions, yield was consistently lower and less responsive to increased N or plant density. These findings suggest that Pivot Bio® has potential as a sustainable nitrogen supplement in irrigated systems, allowing for reduced N application without sacrificing yield