One of the most devastating diseases in the world that affects rice is sheath blight (ShB), caused by Rhizoctonia solani. Management of the sheath blight is carried out through cultural, biological, and chemical applications. Breeding for resistance has been explored. However, due to the pathogen's complex mechanisms, complete resistance in rice cultivars has not yet been achieved. Phenotypic evaluation through epidemiological concepts provides a relevant basis for identifying potential donors of resistance. The resistance is assessed through epidemiological phenotypic quantification of physiological and disease escape. Sixteen selected lines were subjected to a micro-field condition to test for overall resistance. Disease resistance was evaluated based on key parameters, including tiller incidence, relative lesion height, and lesion number. Statistical analysis, including ANOVA and multivariate analysis, identified four lines, Da Nuo, Gie 57, ShB 6, and ShB 5, with moderate to high resistance. Moreover, Oryza rufipogon lines (ShB 4, ShB 5, ShB 6, and ShB 9) demonstrated promising resistance levels, indicating their potential as donors in breeding programs for enhanced ShB resistance. This study provides valuable insights into the quantitative resistance mechanisms of rice genotypes against sheath blight, laying the groundwork for future breeding strategies to mitigate disease impact.