The search and development of new biologically active organic compounds remains a cornerstone of modern medicinal chemistry. Among the promising scaffolds, 6-(5-mercapto-4-phenyl-4H-1,2,4-triazol-3-yl)pyrimidine-2,4(1H,3H)-dione stands out due to its unique combination of heterocyclic moieties. This scaffold not only incorporates the biologically significant 1,2,4-triazole ring—a well-known pharmacophore with antimicrobial, antioxidant, and anti-inflammatory properties—but also integrates the pyrimidine-2,4-dione fragment, structurally related to vitamin B13 (orotic acid), which is essential for nucleic acid biosynthesis. The synergy between these two fragments opens up new avenues for designing compounds with enhanced pharmacological profiles.
In this study, we synthesized a series of novel derivatives based on the core 6-(5-mercapto-4-phenyl-4H-1,2,4-triazol-3-yl)pyrimidine-2,4-dione scaffold by introducing various substituents via S-alkylation and S-arylation reactions, aiming to modulate lipophilicity and electronic properties. These modifications are expected to influence the compounds’ bioavailability and interactions with biological targets. The synthesized derivatives were characterized by spectroscopic techniques, including NMR, to confirm their structures. Preliminary biological evaluations revealed that several compounds exhibited significant antimicrobial activity against both Gram-positive and Gram-negative bacteria, along with notable antioxidant potential in standard DPPH assays.
These results suggest that hybrid molecules combining the triazole-thiol moiety and the vitamin B13-derived pyrimidine-dione core represent a promising class of compounds for further pharmacological development. Ongoing studies are focused on detailed pharmacological profiling, mechanism-of-action investigations, and structure-activity relationship (SAR) analyses to optimize their biological efficacy and safety. The potential applications of these derivatives may extend to antimicrobial therapy, anti-inflammatory treatment, and antioxidant interventions, paving the way for innovative therapeutic agents.
