The genus Anthemis (Asteraceae) is well known for its richness in bioactive secondary metabolites, particularly phenolic compounds, which are associated with notable antioxidant properties. Despite this interest, comparative data on the antioxidant activity of extracts from different Anthemis species remain limited, especially regarding the influence of solvent polarity. This study focuses on evaluating and comparing the antioxidant potential of dichloromethane and hexane extracts from the aerial parts of Anthemis punctata and Anthemis maritima.

In this study, we investigated the antioxidant potential of four extracts obtained from the aerial parts of two Anthemis species: A. punctata and A. maritima. The plant materials were successively extracted using hexane and dichloromethane, yielding ARP1H and ARP1D (A. punctata), and ARP2H and ARP2D (A. maritima). The antioxidant activity was evaluated by the DPPH radical scavenging assay, and IC₅₀ values were determined.

The results revealed notable differences according to both species and solvent polarity. The dichloromethane extract of A. punctata (ARP1D) showed the strongest antioxidant effect (IC₅₀ ≈ 80 μg/mL), followed by the dichloromethane extract of A. maritima (ARP2D, IC₅₀ ≈ 110 μg/mL). In contrast, hexane extracts (ARP1H and ARP2H) exhibited weaker antioxidant activity, as indicated by their higher IC₅₀ values.

Overall, this comparative study demonstrates that solvent polarity and plant species significantly influence antioxidant capacity, highlighting A. punctata dichloromethane extract as a promising natural source of antioxidant compounds.