Dust generation in the mining industry represents a persistent environmental and occupational health challenge, contributing to air pollution, visibility reduction, and respiratory illnesses among workers and nearby communities. This study focuses on developing an innovative and eco-friendly dust suppressant derived from Sargassum crassifolium seaweed and glycerine, aiming to provide a sustainable alternative to conventional chemical-based agents. The experimental investigation was conducted on limestone samples, a material commonly associated with mining operations and known for its high potential for dust emission. The results revealed that the inclusion of glycerine enhanced the viscosity and adhesion properties of the seaweed extract, improving its ability to bind fine particles and prevent airborne dispersion. Among the various formulations tested, the 58% Sargassum–glycerine mixture demonstrated the most effective performance, achieving a balance between fluidity, moisture retention, and biodegradability. This concentration allowed sufficient water infiltration to maintain soil and material moisture, which is critical for reducing dust release and improving resistance to wind erosion. Laboratory evaluations confirmed that the treated samples exhibited a substantial reduction in dust formation compared to untreated controls. Overall, this seaweed-based dust suppressant offers a promising, sustainable solution for mitigating dust pollution in the mining sector, improving air quality, safeguarding human health, and minimizing environmental impact.