Antimicrobial resistance (AMR) is a complex problem that threatens human and animal health and overall global security. In 2019, AMR was listed by the World Health Organization (WHO) as one of the top 10 dangers in the World. AMR is on the rise, and if current trends continue unchecked, up to 10 million fatalities per year could occur by 2050. To address the problems caused by AMR, international organizations, including the World Health Organization, the FAO, and other relevant organizations, have suggested the One Health Approach, which is applicable in multiple sectors. However, despite growing importance, there remains a limited understanding of how One Health principles have been operationalized and integrated within AMR research worldwide. Additionally, there are inadequate reviews on multidisciplinary fields and limited quantitative reviews. This study uses a systematic and bibliometric review to address this gap by evaluating the conceptual structure, performance, and further examining how the One Health Approach is operationalized within AMR research. Relying on the PRISMA protocol, this study analyzes ninety-six (96) papers extracted from the Scopus and PubMed databases from 2010 to 2025 using Vosviewer and R-biblioshiny. The results show that the United Kingdom, the United States, and India are the top three (3) prominent countries in AMR research under the One Health Approach. Ballash, G.A., is the most influential author. Furthermore, the emergent themes of AMR research under the One Health Approach are as follows: One Health policy dimensions and public health, microbiology and mechanisms of bacterial resistance, and pharmacological and clinical studies. The results from this systematic review show that the integration of the One Health Approach has progressed from concept to practice, particularly in the health, veterinary, and environmental sectors. Hence, the study recommends an increase in investment, research, and innovation across all sectors, especially sectors such as agriculture that have received less attention.