Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found commonly in plants, especially legumes. It has some physiological effects on cell metabolism; it induces mitosis and affects the cell membrane regarding transport and permeability to proteins. It agglutinates most mammalian red blood cell types and have the mitogenic effect. This is the reason that PHA is extensively used in the laboratory as well as clinical set up for karyotyping analysis. The downside of PHA use is its cost and storage (-20^oC) resulting into increased cost. We have synthesised acetylated pyrazolines  as anticancer agents and during their evaluation for anticancer potential in normal control cells, we were surprised by their cell proliferation activity. We thought of relating our compound to PHA (PHA mimetic) and performed the basis karyotyping experiment keeping PHA as standard and found our compounds to be PHA mimics. The compounds are thus being evaluated for their further PHA mimetic potential using B/T cell specific cell cycle analysis and karyotyping experiment keeping PHA as standard and found our compounds outstanding PHA in every aspect. The compounds are thus being evaluated for their further PHA mimetic potential.