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Simultaneous determination of albendazole and praziquantel in rat plasma by HPLC-UV
1 , * 2 , 2 , 2 , * 2
1  Sigma Institute of Pharmacy, Woghodia, Vadodara, Gujarat (390016), INDIA
2  Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand (835215), INDIA


A simple, sensitive and easy high-performance liquid chromatographic [HPLC] method has been developed and validated for the simultaneous estimation of albendazole [ABZ] and praziquantel [PRQ] using diazepam as an internal standard in rat plasma. Extraction of ABZ, PRQ and Diazepam in rat plasma was carried out by a process which involves precipitation of proteins called protein precipitation. Protein precipitation method was done by using 8.25% v/v perchloric acid. Chromatographic separations were performed using an Enable C18 column [250 mm × 4.6 mm, 5 μm: Spinco Biotech Pvt. Ltd]. The mobile phase consisted of acetonitrile: water [60:40, v/v] at a flow rate of 1.0 mL min-1 at an ambient temperature for plasma samples. The above method was developed and validated over the range from 50-8000 ng mL-1 for both ABZ and PRQ in the rat plasma. The recovery of ABZ was found to be 97.81 to 105.28%, whereas PRQ recoveries ranged from 96.80 to 102.63% in the rat plasma. Acceptable Intra-day and inter-day assay precision [<15% R.S.D.] and accuracy [<15% R.S.D.] were observed over 50–8000 ng mL-1 for ABZ and PRQ in rat plasma. This method can be effectively applied to the pharmacokinetic study of albendazole and praziquantel in rat plasma to determine all the pharmacokinetic parameters.

Keywords: Albendazole; Praziquantel; Validation; Bioanalytical; HPLC