The cultivation of sugarcane is an agricultural activity of great socio-economic importance in Ecuadorian Amazon. The study was carried out in the sugarcane production area of the municipality of Simon Bolivar, Pastaza province, Ecuadorian Amazon Region.We used a factorial design in random blocks with three replicates for a total of 27 experimental plots.The study factors were the varieties of sugarcane identified as: POJ 27-14, Crystalline and POJ 93 and two types of organic fertilizers: pollinaza and sugarcane bagasse at a dose of 9060 kgha^-1 compared with an absolute control without application of fertilizer.The analysis of combined variance and the Tukey's mean test with a significance level of 5% was performed to detect differences in mean between treatments.The results suggest that the concentration of sugars expressed in brix degrees did not show significant differences between the varieties, but it did show differences between the types of fertilization, being higher with the application of pollinaza with values of 15.9 degrees brix.The agricultural yield was affected according to the variety, registering higher values with variety POJ 27-14 with a yield of 107 t ha^-1 in comparison with the other two varieties, which showed statistically similar yields.