The composition of Hypericum maculatum herb contains a wide range of biologically active compounds: flavonoids (hyperoside, rutin, quercetin), tannins, carotenoids, essential oil, ascorbic acid and others.

Flavonoids and vitamin C have antioxidant, capillary strengthening and venotonic effects, carotenoids exhibit a pronounced regenerative effect.

Two-phase extraction by a system of solvents of different polarity allows for two extracts containing both polar and lipophilic bioactive compounds from one raw material in one technological cycle.

The authors investigated the effect of the non-polar extractant (hexane and almond oil) type and the emulsifier (Tween 80) on the effectiveness of bioactive substances two-phase extraction from the herb Hypericum maculatum. Tween 80 is a non-ionic surfactant from the group of polysorbates. The optimal content of Tween 80 in the extractant system was 1 ml per 1 g of raw material. The ratio of raw materials: polar phase: non-polar phase was 1: 10: 10. The quantitative content of flavonoids was determined in the studied polar phases by spectrophotometry, and carotenoids in non-polar phases.

It was found that flavonoids were more efficiently extracted with a system of extractants of 70% ethanol: almond oil. The addition of an emulsifier significantly increased the release of flavonoids and carotenoids during the extraction process. Tween 80 contributed to an increase in the solubility inside the particles of raw materials of both lipophilic and polar bioactive substances and facilitated their subsequent diffusion into the extractant.