Please login first
Antiviral activity of fluorinated compounds against DNA- and RNA-containing viruses
* 1 , 2 , 1 , 3
1  D.K. Zabolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, Ukraine
2  ESC "Institute of Biology and Medicine" Taras Shevchenko National University of Kyiv, Ukraine
3  Institute of Organic Chemistry NAS of Ukraine

Published: 31 October 2019 by MDPI in 5th International Electronic Conference on Medicinal Chemistry session ECMC-5

Viral infections occupy an important place in human infectious pathology, as one of the most common and poorly controlled infections. At present, Influenza A virus (IAV) and Epstein-Barr virus (EBV) cause different infections, thereby imposing a huge toll on both human health and the economy worldwide. Thus, caused by the influenza virus, influenza or flu is a seasonal disease affecting millions of people in the world every year. The EBV is one of the world's most disseminated viruses and has been associated with a post-transplant lymphoproliferative disease, together with several cancers including Burkitt’s lymphoma, gastric cancer, Hodgkin’s lymphoma, lung carcinoma, nasopharyngeal carcinoma. Therefore, screening new effective drugs is an urgent and important problem.

Our study aimed to determine cytotoxicity and antiviral effect of fluorinated compounds against the Influenza A virus and Epstein-Barr virus. The cytotoxicity was studied in two cell lines (B95-8 and MDCK) by MTT assay. The cytotoxicity of the compounds the rate was 41 – 990 μg/ml in the B95-8 cell culture, which produces EBV, and 200 – 478 μg/ml in MDCK cells.

Compounds 10S-47 and 10S-49 were able to inhibit reproduction of IAV with EC50 (effective concentration inhibiting reproduction of the virus by 50%) of 38 μg/ml and 50 μg/ml, respectively. It was shown that fluorinated compounds showed low effectiveness against the Epstein-Barr virus, and inhibited the reproduction of the virus at 20% in the concentration range of 1 – 100 μg/ml. Also, we studied the potency of compound 10S-47 to make an apoptosis induction because it exhibited a significant cytotoxic effect on the growth of transformed cells. It was established that for compound 10S-47 at 40 mg/ml, the percentage of apoptotic cells exceeded the control and was 10%.

Keywords: Fluorinated compounds, antiviral activity, Influenza A virus, Epstein-Barr virus