The human pathogen of Mycobacterium tuberculosis (MTB) is indeed one of the renowned important longtime infectious diseases, tuberculosis (TB). Interestingly, MTB infection has become one of the world's leading causes of human death. In trehalose synthase, the protein NCGM 946K2 146 found in MTB has an important role. For carbohydrate transport and metabolism, trehalose synthase is required. The protein isn't clarified yet, though. In this research, an in silico approach was therefore formulated for functional and structural documentation of the uncharacterized protein NCGM946K2 146.Three distinct servers, including Modeller, Phyre2, and Swiss Model were used to evaluate the predicted tertiary structure. The top materials are selected using structural evaluations conducted with the analysis of Ramachandran Plot, Swiss-Model Interactive Workplace, Prosa-web, Verify 3D, and Z scores. This analysis aimed to uncover the value of the NCGM946K2 146 protein of MTB. This research will, therefore, improve our pathogenesis awareness and give us a chance to target the protein compound.