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Research of the influence of zinc ions on synthesis and properties of hydroxyapatite
* 1 , * 2 , * 1 , * 1
1  National Research Tomsk State University, Chemical faculty
2  National Research University ITMO

Published: 09 November 2020 by MDPI in 1st International Electronic Conference on Applied Sciences session Materials

Hydroxyapatites modified with metal ions are the main inorganic components of bone tissue that are approved for use as components for biocomposites and coatings for surgical implants. This study examined prototypes of functional materials for bone implants based on hydroxyapatite modified with zinc ions. Zinc is an essential trace element necessary for many biochemical processes. Also zinc ions have antibacterial activity. Zinc-modified hydroxyapatites were obtained by liquid-phase microwave synthesis. By varying the content of zinc nitrate during the synthesis, a line of samples with different amounts of zinc moles (x = 0.1; 0.5; 0.9) was obtained. Elemental analysis was performed before and after the in vivo experiment. The composition and structure of the materials obtained were determined by X-ray phase analysis, X-ray microanalysis, and IR spectroscopy. To determine the antibacterial activity, the determination of the number of cells by Koch's method was used. Escherichia coli served as a test object. In vivo biocompatibility was assessed by subcutaneous implantation of samples into house mice (Mus musculus). As a result of the study, it was found that the obtained samples represent the phase of hydroxyapatite Ca5(PO4)3(OH) hex. Antibacterial activity and biocompatibility have been established. The studied materials have antimicrobial activity, the samples did not cause significant changes both in the internal organs and in the general condition of laboratory animals during the entire experiment. The work was carried out within the framework of a scientific project supported by the TSU Competitiveness Enhancement Program.

Keywords: zinc-modified hydroxyapatite, phase composition, biological activity, surface morphology, cytotoxicity, antibacterial activity