The imidazole ring is a planar heterocycle whose derivatives are applied in several scientific areas, such as Medicinal, Materials and Supramolecular Chemistry. The presence of the imidazole ring in these structures is the key to the development of new drugs, since this heterocycle is present in several naturally occurring biological structures. Therefore, over the past few decades, several imidazole derivatives have been synthesized and occupy a unique position in the field of Medicinal Chemistry due to their diverse biological activities. In order to continue the work developed by the research group, we report the synthesis of 2,4,5-tri(hetero)arylimidazole derivatives and their respective characterization by ¹H and ¹³C nuclear magnetic resonance (NMR) and UV-Vis. spectroscopies. As a complement of the characterisation of the synthesised 2,4,5-tri(hetero)arylimidazole derivatives, a screening for antibacterial activity showed inhibition of Staphylococcus aureus proliferation, suggesting antibacterial activity. Therefore these new compounds have the potential for the development of new drugs.