Diabetes mellitus is an endocrine disorder, characterized by hyperglycemia with abnormal carbohydrate, protein and lipid metabolism. There has been an increase in prevalence of diabetes mellitus, with an estimate of around 171 million people worldwide. Currently available synthetic treatments are associated with toxic side effects, therefore plant sources are considered as an alternative for effective management of diabetes mellitus and its associated complications. Mehon, an antidiabetic polyherbal formulation is available commercially with proven antidiabetic activities however, lack studies on systematic mechanism of action. Therefore, in the present study Hydro-alcoholic(HAE) and aqueous(AQE) extracts of Mehon were analyzed for its Total phenolic, Total flavonoid contents, effects on a-amylase and a-glucosidase inhibitory activities, followed by glucose adsorption, diffusion and transport across membrane of yeast cells. Our results revealed that HAE exhibited higher Total Phenolic content ( 95.44±0.22GAE/mg) and Total flavonoid content (86±0.15QE/mg) compared to AQE and (TPC 47.87±0.29GAE/mg, TFC 38.82±0.15QE/mg).Glucose Adsorption increased remarkably with an increment in glucose concentration (5mM/L-100mM/L), highest glucose binding capacity was observed to be 74.56 ±1.02 mM and 73.86±1.09mM at 100mM concentration for HAE and AQE respectively. Rate of glucose uptake into yeast cells was linear in all the 5 glucose concentrations (5mM to 25 mM). In both the extracts, diffusion of glucose was directly proportional to the time from 30 to 120 min. The GRDI was calculated to be 27.44% and 17.43% at 30 min which gradually reduced to 14.63% and 7.00% at 120 min for HAE and AQE respectively. Among both the extract HAE showed alpha-amylase (71.87%) and alpha-glucosidase (70.12%) inhibitory activities in comparison to standard drugs Acarbose These mechanisms might be accountable in lowering the concentration of postprandial serum glucose and thus confirms the antihyperglycemic propensities of commercial antidiabetic polyherbal formulations Mehon.